Isolation and antibacterial activity of endophytic fungi from citronella grass (Cymbopogon nardus)

Abstract. Fais MM, Yurnaliza Y, Hastuti LDS. 2022. Isolation and antibacterial activity of endophytic fungi from citronella grass (Cymbopogon nardus (L.) Rendle). Biodiversitas 23: 6564-6569. Endophytic are a group functional microorganisms plants that can be used as sustainable source to produce bioactive metabolites including pharmaceuticals. Citronella Rendle) is kind medicinal plant could research the capabilities it harbors. The objective this study was isolate different leaves stem may able substances. bacteria Methicillin-resistant Staphylococcus aureus (MRSA) Escherichia coli ATCC 25922 were in plug agar method determine potency antibacterials. Using ITS1F ITS4R primers, fungal isolates identified molecularly. Result showed total 20 obtained citronella, 15 5 leaves. Three i.e. CNB 254, 253, CND 111, exhibited highly potent antagonistic against tested bacteria. ethyl acetate extract had higher ability than methanol based on diameter inhibition zone formed. three potential molecularly submitted GenBank, such Trichoderma atrobrunneum 253 (OP584477), afroharzianum 254 (OP584478), Fusarium pernambucanum 111(OP584479). results revealed has relationship with include strains exploited antimicrobials.